Destiara Wulan Ramadhani

Universitas Islam Malang, Indonesia

Tintrim Rahayu

Universitas Islam Malang, Indonesia

Gatra Ervi Jayanti

Universitas Islam Malang, Indonesia

DOI: https://doi.org/10.19184/jid.v27i1.60014

ABSTRACT 

Sunlight emits light dispersed into a spectrum ranging from blue to red wavelengths. Blue light has a wavelength of 450–495 nm, while red light ranges from 620–750 nm and generates higher temperatures than normal light conditions. This study evaluated the effects of 2-iP, light spectra, and NBs N₂ as protective agents to maintain planlet surface integrity under spectrum exposure. Planlets were cultivated using a thin-layer system by temporary immersion in MS medium within thin-wall and glass chambers. All treatments were placed on racks equipped with spectrum and covered with black fabric to block external light. Blue, red, and white spectra with NBs N₂ produced the best growth in the thin-wall, with an average weight increase of 0.24 g and height increase of 1 cm. The highest shoot proliferation occurred under blue light without NBs N₂, producing an average of 9 shoots, while white light with NBs N₂ produced up to 10 new shoots. All planlets in the thin-wall system survived and grew optimally, indicating that NBs N₂ maintained medium stability and enhanced nutrient uptake despite elevated temperatures. In contrast, no growth occurred in glass chambers due to excessive heat accumulation, causing complete planlet desiccation by the 12th DAT.

Keywords: Color Spectrum, Dendrobium sp, Nanobubbles (NBs), Nitrogen, Chambers..